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Future studies are needed and will hopefully replicate our results, involve a larger sample size, have a control arm, and include a longer follow-up with the goal of continuing to improve MGD treatment, enhance patients quality of life, and help relieve some of the long-term healthcare burden related to ocular surface diseases in the United States

Statistical significance was assessed in GraphPad Prism and tested by one- or two-way ANOVA for repeated measurements followed by Tukeys multiple comparison test, or unpaired one-way ANOVA followed by Tukeys multiple comparison test

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Negative side effects may include a paradoxical decrease in energy levels (most likely due to detoxification from methylation) or slight pain at the injection site which diminishes over 5-10 days

Vitamins and Hormones , vol