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2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

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[DOI] [PubMed] [Google Scholar] Fersht A

Second, it is a gastric effect achieved through the brain , via vagal outflow to the stomach, not through amylin acting locally on gastric tissue

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