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For C3 antibody staining we used vibratome-cut sections

found that exogenous -KG mimics glutaminase-mediated ferroptosis, while using a transaminase inhibitor, amino-oxyacetate (AOA), reverses ferroptosis [85, 86]

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Necroptosis of erythrocytes relies on the RIPK1 (receptor-interacting serine/threonine-protein kinase 1)/RIPK3 (receptor-interacting serine/threonine-protein kinase 3)/MLKL (mixed lineage kinase domain-like pseudokinase), similarly to nucleated cells

These technologies potentially allow the setup of an in vitro test system, which resembles the in vivo situation as closely as possible 27