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Intracranial injections were performed under deep anaesthesia using vaporized isoflurane (22.5%) and oxygen (2 l min 1 )

They utilized dark pretreatment and found that the expression of both MpGSTZ1 and MpGSTU2 remained unchanged following transfer to the darkness, whereas the expression of MpGSTU1 and MpGSTF1 decreased by ~50 and 75%, respectively, when plants were placed in the dark for 2 h (Dean et al., 2003)

Nat Rev Dis Primers 11 , 51 (2025)

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AANA J 83:425433 Bushra R, Aslam N (2010) An overview of clinical pharmacology of ibuprofen