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Materials and methods Cell lines and reagents The MCF-7 human breast cancer cell line was obtained from Dr Dean Edwards (University of Texas, San Antonio, TX, USA) and was maintained in phenol red RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/mL penicillin, 100 g/mL streptomycin, 1 non-essential amino acids and bovine insulin at 6 ng/mL

GHK-Cu topical dosage: serums, creams and scalp solutions Topical GHK-Cu is where most patients start - especially for facial rejuvenation and early scalp support

Additional tests may include full blood count (looking for macrocytic anaemia), methylmalonic acid, and homocysteine levels

Visual signs of degradation can include cloudiness or a change in color in the solution

Hydration plays a role too