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50 mM potassium phosphate buffer (pH 7.0), 0.5 mM ascorbic acid, 0.1 mM EDTA, 0.1 mM H 2 O 2 , 0.1 mL enzyme and water to make a final volume of 3.0 mL in which 0.1 mL of H 2 O 2 was added to initiate the reaction