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Briefly, approximately 1.510 5 host cells (A549, RAW 264.7, PBL-985, and PBL-985 nox2 -KO cell lines) were seeded per well in 12-well plates and cultured in DMEM + 5% FBS at 37C and 5% CO2 for 24 h until reaching 95% confluence, equivalent to approximately 3 10 5 cells

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The demand for Se will increase after suffering environmental pressures, and supplementation is a necessary condition to meet the needs of fish growth ( 4.1 Harmful effects of selenium excess Se is an essential micronutrient required for fish, which plays an important role in the antioxidant system and also affects the lipid metabolism, sugar metabolism and amino acid metabolism of aquatic animals (Yoboue et al., 2018