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When the barrier is compromised through over-exfoliating, environmental stress, or simply dry conditions skin starts to feel rough, tight, or reactive

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Intracellular ROS and lipid peroxidation level Reactive oxygen species (ROS) were measured on the basis of the intracellular peroxide-dependent oxidation of DCFH-DA (S0033S, Beyotime) to form the fluorescent compound 2,7-dichlorofluorescein (DCF)