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Slides were also counterstained with mouse anti-insulin primary antibody (1:1000, 4 C overnight) and goat anti-mouse secondary antibody conjugated with Alex Fluor 594 (1:400, 1 h at room temperature)

Peripheral blood mononuclear cells (PBMCs) are a diverse group of immune cells including lymphocytes (T cells, B cells, and NK cells) and monocytes, that play a role in immune function and inflammation

Unless otherwise indicated, cell viability was assessed 6d after plating, using the AQueousOne Solution Cell Proliferation Assay (Promega)

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doi: 10.1016/j.intimp.2013.03.020 154 WenZJinKShenYYangZLiYWuBet al