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In fact, since the cells of the gut will see the highest doses of metformin they will experience the greatest level of inhibited complex I which may explain the gastrointestinal side effects (nausea, diarrhea, anorexia) of the drug that limit its utility in many patients

Water inflow, water outflow, and changes in water storage are not considered

A typical transitional protocol might involve four weeks at 0.25 mg daily, followed by two weeks at 0.15 mg daily, then maintenance at 0.1 mg daily for extended periods

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Blocked cells on coverslips were then incubated overnight in a CHAC1 primary antibody (1:500 in 3% BSA/PBS