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The volume of BAC water is selected to achieve the desired concentration for a particular laboratory protocol

10.1093/humrep/deab190 53 Romero-ReyesJ.CrdenasM.Damin-MatsumuraP.DomnguezR.AyalaM

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Liquid-liquid extraction, either with acetonitrile based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) protocols or by salting-out assisted extraction with ethyl acetate are other commonly used sample preparation techniques used also for large cohorts (Njumbe Ediage et al