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Lorillard quickly filed a second suit against Legacy and also filed suit against the National Association of Attorneys General and the attorney general of Delaware, contending that they were responsible for Legacy's actions

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The PCR conditions for the 16S V3-V4 rRNA gene were an initial denaturation step at 95C for 5 minutes, followed by 25 cycles of 95C for 30 seconds, 50C for 30 seconds and 72C for 40 seconds, and a final extension step at 72C for 7 minutes