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The cel ls were pelleted after 20 hours by centrifugation using a Fiberlite F9-61000 rotor in a Sorvall LYNX 6000 Superspeed centrifuge at 2,000 g for 20 minutes, re-suspended in 40 ml lysis buffer containing 50 mM Tris, pH 7.5, 150 mM NaCl, 20 mM imidazole, 20 % glycerol, 2 mM TCEP, 250 units of Benzonase Nuclease and bacterial Protease Inhibitor Cocktail (Amresco

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10.1371/journal.pone.0033592 PLoS One

Paclitaxel reduces tumor growth by reprogramming tumor-associated macrophages to an M1 profile in a TLR4-dependent manner

As a result, -tocopherol with three methyl groups is the most active of the tocopherols and -tocopherol with one methyl group is the least active (Ungurianu et al., 2021)