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To analyze the effect of GSH on the activity of the pFeSAN nanozyme, 20 L of pFeSAN or GSH-pretreated pFeSAN (1 mg/mL), 20 L of TMB (50 mM) and 960 L of HAc-NaAc buffer (pH 4.0) were mixed and incubated at 25C for 10 min

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While not one of the more severe GHK-Cu contraindications, its a safety factor to consider, and any use should start at a very low dose with careful monitoring for dizziness or lightheadedness