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doi: 10.1096/fj.202100085R 154 WangYShiPChenQHuangZZouDZhangJet al

Kuwait Univ

After incubation at 4 C for 30 min, the reaction mixtures were added to 100 L of 50% (v/v) Ni-NTA superflow resin slurry that was pre-equilibrated with buffer containing 50 mM BisTris, pH 6.8, 100 mM NaCl, 25 mM imidazole, and 10% glycerol