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The cells were grown in a defined medium containing DMEM/HAM F12, supplemented with 33 mM glucose, 2 mM glutamine, 100 U/ml penicillin, 100 g/ml streptomycin, 13 mM sodium bicarbonate, 5.5 g/ml transferrin, 10 g/ml insulin, 1 pM -estradiol, 3 nM triiodothyronine, 20 nM progesterone, 5 ng/ml sodium selenite, and 100 M putrescine

Because both protocols use a before-bed injection on a 5-days-on/2-days-off schedule, they run cleanly in parallel with no scheduling conflict

Lipolysis not only in the context of obesity, but also body composition optimization, including in bodybuilding studies