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PC-3 cells, which were transfected with luciferase reporters (Luc-1-Luc-6) or plasmids (FBI-1, siFBI-1, or siSP1), were harvested for luciferase assays using the kits (Promega Corporation, Madison, WI, USA) according to the instructions provided by the manufacturer and methods described in previous publications [41,44,45]

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Additionally, HCQ and CQ can bind to nucleic acids within the endosome, preventing the interaction of endosomal TLRs with their ligands and thereby inhibiting subsequent TLR activation

[35] Women who are pregnant, or planning to be pregnant, must consult their physician regarding the use of lipotropic injections for weight loss

We also investigated immunohistochemistry analysis of CD4+ T cell, PLC and I B phosphorylation and evaluation of cytokines level in the spleen, which is one of the originated organ of exogenous T cells