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We provide neutral educational insights into tobacco brand categories, blend characteristics, and regulation differences

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Participants also showed reductions in self-reported cigarette dependence (-45%), smoking frequency (-29%), and smoking intensity (-78%

Paraffin blocks were sectioned onto SuperFrost Plus slides, dried overnight, and baked for 1 h at 60 C before immediately proceeding with the RNAScope Multiplex Fluorescent v2 assay according to the manufacturers instructions with the following modifications: Target retrieval was performed for 15 min in a boiling beaker, Protease III was used for 30 min pre-treatment at 40 C and hybridized slides were left overnight in 5 SSC before proceeding with the amplification and labeling steps

Cigar smoking is relatively common