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Hangzhou, China: Zhejiang Gongshang Univ

The specific methodology is as follows: Newly acquired mice were acclimatized in the animal facility for at least one week, then placed on a metal grid for 12 h each day in the experimental room (8:00 AM-8:00 PM), and the fecal output was recorded

Additionally, levels of GPX4, an important regulator of ferroptosis, are reduced in spinal cord neurons of both MS brains and experimental autoimmune encephalomyelitis (EAE) mice, indicating that ferroptosis is an early pathological event in EAE [190]

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Several other studies have demonstrated the safety of this method [322, 324, 326, 327]