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The pBDMs cell suspension was centrifuged, and the cell pellet was resuspended in fresh triple-culture medium (2 parts of AX E2 Alveolar Barrier Medium: 1part of RPMI) and added on the apical side at a ratio of 1:7 (pBDMs: AX iAECs)

Study link: Centers for Disease Control and Prevention: Smoking and Stroke Research article: Smoking and Risk of Stroke: Systematic Review and Meta-Analysis This review found that smoking significantly increases stroke risk through vascular damage, atherosclerosis, and enhanced blood clotting

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[26] [28] Perry later attributed his behavior to his feeling like a family outsider who no longer belonged once his mother began having children with Morrison