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Each year, smoking causes more than 8 million deaths and remains one of the most serious public health threats the world has ever faced

After protease digestion and centrifugation (1200 rpm, 5 min), PBECs were cultured in bronchial epithelial cell medium (#3211, ScienCell, San Diego, California, USA), supplemented with 100 U/ml penicillin, 100 g/ml streptomycin and 1 bronchial epithelial cell growth supplement (#3262, ScienCell, San Diego, California, USA) using poly-L-lysine-coated flasks and employed for experiments at passage 24 without mycoplasma contamination (Additional file 2: Fig

Our intention is for this collection to remain actively curated as ever more genomic data are produced and a wide range of phenotypic data are generated not only by us, but also by the community

What led up to that moment

During these years, many young people arrived from the South