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Data analysis Unsupervised principal component analysis (PCA) was first used to observe the overall distribution of samples and the stability of the analysis, followed by supervised partial least-squares discriminant analysis (PLS-DA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) used to distinguish the differences in metabolic profiles

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They found that non-smokers who began using heated tobacco products were 5.8 times as likely to subsequently transition to smoking within the six month study period as people who had never used heated tobacco products