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A total 0.1 10 6 cells were collected and incubated with 100 l Annexin V binding buffer along with 5 l of fluorescein isothiocyanate conjugated Annexin V (Life Technologies, USA) and 5 l of propidium iodide (Life Technologies, USA) for 20 min at room temperature

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This solution must be used in a well ventilated area because ammonia fumes are harsh on the respiratory system

Dr Ali Al Ameri, in his post, put out a plea to Dubai Municipality to address the issue, while citing a recent report about similar complaints from residents and citizens

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