Figure 1 Materials and methods A search of scientific literature was conducted using the PubMed database ( The following Medical Subject Headings (MeSH) terms were used: e-cigarettes AND flavor AND nicotine AND e-liquid AND cytotoxicity AND cellular damage and health AND adolescent AND young people. We included publications from 2020 to 2025 because fourth-generation electronic cigarettes appeared on the market in 2020 ( in vitro studies, in silico models, and studies using cannabis cigarettes or tobacco flavors, hookahs, nicotine pouches, and waterpipes
1)Causethesamecarmodelmaybedifferentlyproducedindifferentcountries
European vaping laws outlined by the Tobacco Products Directive (TPD) prohibit the use of diacetyl, an artificial flavouring, in vape liquids
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Long-term use has been linked to cannabis use disorder (CUD), mental health disorders, and potential risks associated with smoking, such as respiratory problems (23)