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Circadian variation affects baseline measurements

After washing steps, the variety was imaged using the Illumina iScan SQ instrument to capture raw image intensities, enabling further analysis

However, previous studies have indicated that some antibodies used in ELISA kits available on the market for quantifying pro- or mBDNF may exhibit low specificity, often failing to distinguish between both isoforms 31,32

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It's also needed for energy production in the mitochondria 20 , which can be essential for fertility