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Tobacco is labor-intensive, requiring over a full year of labor to gather the tiny seeds, grow them early in the year in cold frames, transplanting to the outdoor fields when the soil is warm, weeding throughout the summer, "topping" most plants (to remove the flowers and force more of the plant's energy into the leaves), then harvesting the leaves individually for several weeks as they ripen in late Summer-early Fall

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These reactive oxygen species (ROS) oxidize chloroplast lipid membranes and alter chloroplast envelope, increasing ion leakage

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