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Baseline adjustment was performed by subtraction of the pre-existing nicotine concentration from each nicotine concentration obtained after test product administration in that period/day for each subject using the following equation: where C t is the adjusted concentration at time t, C t unadjusted is the observed concentration at time t, C 0 is the pre-product use concentration (5 min), Kel = ln(2)/t, t is 2 h (approximate nicotine half-life), t is the actual sampling time since product administration, and t1 is the actual sampling time since the time of the pre-product use sample

doi: 10.1038/s41598-021-86404-x

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