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The concentration of the digested plasmids was quantitated spectrophotometrically, and 750 ng of each plasmid was then used in a T7 in vitro transcription reaction according to the manufacturers protocols (Ambion T7 mMessage mMachine

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Memetic images are essentially of two kindsentrenched or non-entrenched, but most (if not all) of them use representations of body postures and facial expressions