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Here, we have reconstituted, in vitro and in planta , a minimal nicotine biosynthetic cascade, forming ( S )-nicotine ( S )-( 1 ) from nicotinic acid ( 2 ) and N -methylpyrrolinium ( 3 )

However, the long-term health consequences of prolonged use remain a subject of ongoing research

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Heart Disease : Smoking contributes to heart attacks, strokes, and high blood pressure by narrowing blood vessels and reducing oxygen flow