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After three wash steps utilizing PBS, HRP-linked goat anti-mouse/anti-rabbit IgG (1:4000, MDL Biotech) was used to probe blots at room temperature for 1 h, after which enhanced chemiluminescence was utilized to detect protein bands

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However, the ultimate population impacts of ECs also depend on their possible detrimental effects, especially from long-term and cumulative use

Kamboj A et al (2016): Pediatric Exposure to E-Cigarettes, Nicotine, and Tobacco Products in the United States