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In PTZs, substitutions at the C-10 position, such as alkyl or aryl groups, reduce activity, whereas modifications at the C-2 position significantly enhance potency, such as featuring a 2-phenyl-methyl piperazine at C-2, which demonstrated remarkable activity with an EC50 of 0.5 nM in erastin-induced ferroptosis in HT-1080 cells (Yang et al., 2021)

At 20mg/mL concentration, an 8mg dose requires only 40 units and a 12mg dose requires 60 units, both easily measurable

This helps achieve maximal cellular betaine concentration to allow this molecule to maintain optimal recycling of homocysteine back to methionine while at the same time provides optimum cellular osmotic maintenance to maximize energy sparing