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Apoptotic and live cells were determined by FACS analysis using Annexin V-FITC staining

Rub well into the skin back and forth along the pulse several times

doi: 10.1016/j.jgr.2016.08.004 92 ChenZZhangZLiuJQiHLiJChenJet al

Viscardi et al [1-6], who successfully developed a Nycodenz gradient technique, emphasised the importance of the non-toxic, non-invasive properties of this gradient medium and pointed out that although Percoll gradients had been used previously for purifying these cells, the potentially toxic nature of a polyvinyl-pyrollidone-coated silica colloid was of considerable concern in studies of their function