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Plasmid constructs The short hairpin RNAs (shRNAs) targeting human p63 or ATF4 was generated by inserting specific oligoes into a pLKO.1-puromycin lentiviral vector (10878, Addgene, Cambridge, MA, USA)

Following a final saline wash, 50% glycerol was added to the wells, and bright-field images were taken using an inverted microscope

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Nanomaterials, such as mesoporous zinc oxide, are being explored for topical applications, while smart nano-delivery systems targeting the fungal microenvironment represent a promising frontier (Thapliyal et al., 2025)