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Another embodiment of the invention, wherein tobacco fortified with 2 to 4 mg of nicotine increases the nicotine delivery without increasing the level of p-BSQ

Yet another embodiment of the invention, wherein the mainstream smoke solution is incapable of producing significant oxidative damage to guinea pig lung microsomal proteins in vitro One more embodiment of the invention related to use of nicotine fortified tobacco, which results in increase in delivery of nicotine without increasing the level of p-BSQ

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The preparation of FSCF samples and collected LCF samples included three stages of remained tobacco separation, plug wrap and wrapping paper separation, and filter cutting