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Immunofluorescence Analysis The cells were fixed for 30 min at room temperature with 4% of paraformaldehyde in 0.1 M PBS, 40 pH 7.4, and permeabilized with 0.1% of Triton1-X100 in PBS for 10 min, followed by blocking with 5% skimmed milk in PBS for 30 min

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Curr Med Chem (2015) 22:423456

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10.1161/CIRCULATIONAHA.113.002654 148

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The role of pulmonary surfactants in the treatment of acute respiratory distress syndrome in COVID-19

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