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Electronic Cigarettes and Health Long-term epidemiological studies of the individual and public health impact of e-cigarettes are challenging because these products have evolved rapidly since their emergence in the mid 2000s

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We also observed a trend of increased gene expression of involucrin and TG1 which correlated with their elevated protein levels in the epidermis of smokers, although these results did not reach statistical significance

A second group received vapors with only solvents but no nicotine