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Precursors for MS 2 were filtered by monoisotopic peak determination for peptides, intensity threshold 5.0e 3 , charge state 27, and 60 s dynamic exclusion after one analysis with a mass tolerance of 10 ppm

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Because many of the datasets included UK Biobank participants, who are more educated, lead healthier lifestyles, and have fewer health problems than the UK population 53 , this discrepancy may limit the applicability of our findings to other populations

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