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However, three of those regulators noted that despite these benefits, there are challenges to using this tool including that it can be time consuming

In contrast to sugar, European settlers could make a profit growing tobacco with smaller slaveholdings and less labor exertion

The introduction of tobacco in Europe and its limited accessibility made it a luxury, leading to its association with the elite

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Therefore, some researchers use homemade ALI instruments to obtain in vitro data, making comparison of results even more challenging, as the puffing profiles and exposure conditions may not be identical, leading to changes in aerosol composition, characteristics, and biological responses