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Symptoms at later stages include blood in the urine, persistent pain, weight loss, fever, a lump in the back, and fatigue

Fermentation takes between four and six weeks to complete

It was later discovered that tobacco-specific nitrosamines were the main carcinogenic compound of cigarette smoke [45]

These missions are carried out in collaboration with many national and international tobacco control actors and in close relationship with the French Ministry of Health.

Primer sequences were designed using Primer3 plus from assembled contigs and subsequently used in this study Additional file 10 (download TXT Figure S10