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The cells were maintained in RPMI-1640 medium supplemented with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin (Life Technologies/Gibco, Oshima, New York), in an incubator at 37 C and 5% CO 2

Intermittent exposure to whole cigarette smoke alters the differentiation of primary small airway epithelial cells in the air-liquid interface culture

Desensitization of 5-HT1A receptors by 5-HT2A receptors in neuroendocrine neurons in vivo

Parental smoking increases risk of ear infections, asthma, respiratory symptoms, and impaired lung function

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