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The eluents for the negative polarity mode were eluent A (5 mM ammonium acetate, pH 9.0) and eluent B (Methanol).The solvent gradient was set as follows: 2% B, 1.5 min

Deconvolution and spectra processing was performed using Mass Spectrometry-Data independent Analysis software (MS-DIAL) 58

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Farrell had a big grin as she asked him if the tires needed changing