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Ultrathin sections (50 70 nm) were obtained using an ultramicrotome (EM UC7, Leica, Wetzlar, Germany), double-stained with uranyl acetate and lead citrate, and observed under a transmission electron microscope (JEM 1200EX-II, JEOL, Tokyo, Japan)

Property managers also use them to address smoke or pet odors in apartments, and vehicle detailing professionals often treat car interiors, buses, and RVs with persistent smells from tobacco or food

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Unfortunately, not all cigars can be resuscitated to their former glory once the tobacco has been compromised

There are, however, still some gaps in our knowledge of cardiovascular disease, smoking, and the predictive use of such markers