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After centrifugation, the lung blades were stained with a Panotic Quick kit (Labourclin, Pinhais, Paran) for later microscopic analysis 9

Given that IL-1RA competes with IL-1 for binding to the IL-1 receptor and prevents IL-1-induced pro-inflammatory signalling we wondered if the increased production of IL-1RA in smokers was associated with high IL-1 levels