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For immunofluorescent staining, after incubated with primary antibodies, the sections were incubated with fluorescent secondary antibody Goat anti-Mouse IgG 488 (A11001, Thermo Scientific), Goat anti-Mouse IgG 594 (A11005, Thermo Scientific), Goat anti-Rabbit IgG 488 (A11008, Thermo Scientific), Goat anti-Rabbit IgG 594 (A11012, Thermo Scientific) for 1h at room temperature

In the film, she sang the song with which she would be constantly identified afterward, "Over the Rainbow." She frequently used an excerpt from it as her entrance music during later concerts and television appearances and named it her favorite of all the songs she had ever recorded, showing how "inextricably linked" it was to her career