Membranes were treated with 5% BSA in PBS-T (PBS with 0.1% Tween 20) blocking buffer for at least 1 h at room temperature, then incubated with primary antibody diluted in blocking buffer overnight at 4C
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Nitrogen dioxide may also cause health issues more or less similar to carbon monoxide, such as dizziness, nausea, headache, loss of concentration, etc
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Every profession has any number of myths and misnomers, and dentistry is no different