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The feedback inhibition of IGF-1 on further GH secretion stayed intact, preventing the GH excess that drives insulin resistance with direct injection

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This is one of a series of commentaries under the banner 50 years forward, giving personal opinions on future perspectives in diabetes, to celebrate the 50th anniversary of Diabetologia (19652015)

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For the untargeted experiment, proteins were digested with 0.7 g of trypsin (sequencing grade, Promega, Madison, WI) and 0.7 g of Lys-C (New England Biolabs) for 1 h at 37 C

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