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Midwakh comprises a smoking pipe of Arabian origin and a mixture of tobacco from Iran

The target sample size was 636, based on power analysis for the primary research question using G*Power, with 90% power to determine an effect size (f2) of 0.02 on our primary outcome at an alpha level of 0.05

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The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication

Oxidative stress negatively affects human sperm mitochondrial respiration