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Dimethyl sulfoxide diluted in a .01 M glycine buffer was added to solubilize the cell membranes and the absorbance of reduced MTT in the buffer was quantitated at 590 nm using a plate reader (Biotek Synergy 2, Winooski, VT)

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doi: 10.1042/BJ20020533

One of the biomarkers is 8-hydroxy-2-deoxyguanosine (8-OHdG), generated by the oxidation of the deoxyguanosine at the C-8 position (Figure 1)