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In vitro models to study confinement-induced responses include micropatterned lines [151], 2D micropatterned substrates [85], uni-axial compression [152], polydimethylsiloxane [153,154,155,156]- or polyacrylamide [90]-based microchannel devices, microniches [157, 158], and natural hydrogels [148, 159]

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For most of them (e.g., the cardiac glycosides), the clinical development will be limited due to their toxicity associated with expected side effects in human

That suggests it could be useful as a complementary therapy when these other medications are prescribed

This includes sterility testing, endotoxin limits, pH specifications, and physical characteristic requirements