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Western blotting HTMCs were grown in the above-mentioned TMC medium in five 12-well plates at a density of 5 10 4 /ml for 24 h, and the medium was replaced with a new one, containing 1 l of water (negative control), TGF-1 (20 g/ml) or glucaric acid (10 mM) for 10 min

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In 2 retrospective studies of ketamine for the treatment of refractory headache, which included individuals with chronic migraine, continuous subanesthetic doses of intravenous ketamine reduced headache severity acutely when dosed from 0.1 mg/kg/h to a maximum of 1 mg/kg/h titration based on drug tolerability